Males leave their burrows and wander in search of females during summer/autumn.

The spiders are mainly active at night, as typical daytime conditions would dehydrate them. During the day, they seek cover in cool, moist hideaways. After heavy rain, spider activity is increased, as their burrows may be flooded. When threatened or provoked, funnel-web spiders will display aggressive behaviour, rearing up on its hind legs and displaying their fangs. When biting, the funnel-web spiders maintain a tight grip on their victim, often biting repeatedly.

Sydney funnel-web spiders are mostly terrestrial spiders, favouring habitats with moist sand and clays. They typically build silk-lined tubular burrow retreats with collapsed ‘tunnels’ or open ‘funnel’ entrances from which irregular trip-lines radiate over the ground. In some exceptions, which lack trip-lines but may have trapdoors, the silk entrance tube may be split into two openings, in a Y or T form. Sydney funnel-webs burrow in sheltered habitats where they can find a moist and humid climate, for instance under rocks, logs or borer holes in rough-barked trees. The burrow may be in the hollow of a tree trunk or limb, many metres above ground level.

The female funnel-webs spend most of the time in their silk-lined tubular burrow retreats. When potential prey, which includes insects, lizards or frogs, walks across the trip-lines, they rush out, subduing their prey by injecting their venom. Males, recognized by the modified terminal segment of the palp, tend to wander during the warmer months of the year, looking for receptive females to mate with. This makes encounters with male specimens more likely as they sometimes wander into backyards or houses, or fall into swimming pools. The spiders can survive such immersion for up to twenty-four hours, trapping air bubbles on hairs around their abdomen.

Funnel-web spider venom contains a compound known as atracotoxin, an ion channel inhibitor, which makes the venom highly toxic for humans and other primates. However, it does not affect the nervous system of other mammals. These spiders typically deliver a full envenomation when they bite, often striking repeatedly, due to their defensiveness and large chitinous cheliceral fangs. There have been no reported cases of severe envenoming by female funnel-web spiders, which is consistent with the finding that the venom of the female specimen is less potent than the venom of their male counterparts. In the case of severe envenoming, the time to onset of symptoms is less than one hour, with a study about funnel-web spider bites finding a median time of 28 minutes. This same study revealed that children are at a particular risk of severe funnel-web envenoming, with 42% of all cases of severe envenoming being children. There is at least one recorded case of a small child dying within 15 minutes of a bite from a Sydney funnel-web spider, though that event occurred before the development of antivenom.

Photo  and information are provided by WR Gay Pest Control Pty Ltd  and Bayer.